Peptide Testing

We offer both routine (batch release) and research analytical support for peptide testing, from basic
purity analysis to full spectral analysis and hydrolyzed amino acids. We have full chromatography and
spectroscopic capabilities in-house, including the ability to analyze for impurities such as residual
solvents and heavy metals.

Routine analysis for purity, potency and identity of known or common/commercially-available peptides using
HPLC-UV and HPLC-MS.
- Purity is calculated from HPLC-UV data obtained at 215 nm and is reported as peak area %. Purity
calculations do not include excipients such as salts and buffers and do not include water of hydration.
- Potency is calculated by external calibration against known calibration standards and requires the
availability of reference materials.
- Identity of the peptide for known sequences is confirmed by HPLC-MS with back calculation of the
apparent mass from the spectrum obtained.

Analysis of unknown/research peptides
- Purity is calculated from HPLC-UV data obtained at 215 nm and is reported as peak area %. Purity
calculations do not include excipients such as salts and buffers and do not include water of hydration.
- Mass of the peptide is confirmed by HPLC-MS with back calculation of the apparent mass from the
spectrum obtained.
- FT-IR spectra to aid in spectral interpretation/identification, UV-visible spectrum for ratio calculation
- Hydrolysis/amino acid analysis if sufficient material is available and with some prior knowledge of
the peptide sequence.

Analysis of process contaminants and excipients
-Heavy metals/residual process impurities by ICP-MS
- Residual solvents by GC-MS
- Salts and buffers by ion chromatography and/or ICP-OES